Tell us more. Was the final answer of the question wrong? Were the solution steps not detailed enough? Was the language and grammar an issue? Didn't find yours? Ask a new question Get plagiarism-free solution within 48 hours. Review Please. Next Previous. Related Questions. What tissue forms the model for endochondral ossification? Cartilage B. Fibrous membrane C. We isolated osteoclasts from rat bone marrow and cultured them on bovine bone slices for 48 hours to create resorption lacunae.
After removing osteoclasts, confluent differentiated primary osteoblast cultures were trypsinized and the cells were replaced on the resorbed bone slices for up to 14 days. Our data suggest that after osteoclastic bone resorption, osteoblast-like cells, not macrophages, remove the remaining organic matrix in the lacuna.
After cleaning the lacuna, osteoblast-like cells deposit new collagen fibrils at the bottom of the lacuna and calcify the newly formed matrix only, as visualized by labeled tetracycline accumulation merely in the lacuna during the osteoblast culture. Furthermore, an electron-dense layer rich in osteopontin separates the old and new matrices suggesting formation of the cement line.
Calcif Tissue Res — Calif Tissue Res — Raina V Normal osteoid tissue. J Clin Path — J Pathol — Owen M The origin of bone cells in the postnatal organism. Arthritis Rheum — Vaugan J The physiology of bone. Oxford University Press, Oxford. Immunobiol — CAS Google Scholar. Mineral Electrolyte Metab —9. Bonucci E New knowledge on the origin, function and fate of osteoclasts.
Clin Orthop — PubMed Google Scholar. Chambers TJ Phagocytosis and trypsin-resistant glass adhesion by osteoclasts in culture. Radiol Res — Calcif Tissue Int — J Cell Biol — Chambers TJ Phagocytic recognition of bone by macrophages.
Pathology —7. Mundy GR, Yoneda T Monocytes: interactions with bone cells and lymphocytes during bone resorption. Excerpta Medica, Amsterdam, p Fallon MD in press Osteoblasts mediate osteoclastic bone resorption in cell culture. Holtrop ME, Raisz LG, Simmons HA The effects of parathyroid hormone, colchicine, and calcitonin on ultrastructure and the activity of osteoclasts in organ culture.
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Woods CG The relationship of osteoclasts to collagen fibre orientation. Jaworski ZFG Parameters and indices of bone resorption.
Armour Montague, Paris. Eriksen EF, Melsen F, Mosekilde L Reconstruction of the resorptive site in iliac trabecular bone: a kinetic model for bone resorption in 20 normal individuals. Abstracts of the 17th European Symposium on Calcified Tissues. Calcif Tissue Int 35 Suppl :A8. J Anat — In: Recker RR ed Bone histomorphometry: techniques and interpretation.
Br J Exp Pathol — Chambers TJ, Bristow K, Athanasou NA in press The effect of calcitonin prostaglandins and parathyroid hormone on the cytoplasmic spreading of osteoclasts. Figure 2. Figure 3. Bone Lining Cells Bone lining cells are quiescent flat-shaped osteoblasts that cover the bone surfaces, where neither bone resorption nor bone formation occurs [ 50 ]. Osteoclasts Osteoclasts are terminally differentiated multinucleated cells Figures 4 a — 4 d , which originate from mononuclear cells of the hematopoietic stem cell lineage, under the influence of several factors.
Figure 4. Figure 5. Extracellular Bone Matrix Bone is composed by inorganic salts and organic matrix [ ]. Interactions between Bone Cells and Bone Matrix As previously discussed, bone matrix does not only provides support for bone cells, but also has a key role in regulating the activity of bone cells through several adhesion molecules [ , ]. Local and Systemic Factor That Regulate Bone Homeostasis Bone remodeling is a highly complex cycle that is achieved by the concerted actions of osteoblasts, osteocytes, osteoclasts, and bone lining cells [ 3 ].
Bone Remodeling Process The bone remodeling cycle takes place within bone cavities that need to be remodeled [ ]. Endocrine Functions of Bone Tissue The classical functions of bone tissue, besides locomotion, include support and protection of soft tissues, calcium, and phosphate storage and harboring of bone marrow. Conclusions The knowledge of the structural, molecular, and functional biology of bone is essential for the better comprehension of this tissue as a multicellular unit and a dynamic structure that can also act as an endocrine tissue, a function still poorly understood.
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